Effect of moxibustion on expression of tgf-β and smad4 in colonic mucosa of rats with intestinal fibrosis in crohn disease
Item
Title
Effect of moxibustion on expression of tgf-β and smad4 in colonic mucosa of rats with intestinal fibrosis in crohn disease
Description
J Acupunct Tuina Sci (2012), Huan-gan, Wu
Abstract
Objective: To observe the effect of moxibustion on the expressions of transforming growth factor-β (TGF-β) protein, and Smad4 protein and mRNA in rats with intestinal fibrosis in Crohn disease (CD), and to discuss the action mechanism of acupuncture-moxibustion in treating intestinal fibrosis in CD from the perspective of TGF-β/Smads signal pathway. Methods: A CD intestinal fibrosis model was developed by using male SD rats of clean grade. The rats were randomized into a normal group, a model group, a mild moxibustion group, an electroacupuncture (EA) group, and a herb-partitioned moxibustion group. The rats in the normal and the model groups did not receive any interventions. Tianshu (ST 25) and Qihai (CV 6) were selected for the rats in the mild moxibustion group, the EA group, and the herb-partitioned moxibustion group. Mild moxibustion, EA, and herb-partitioned moxibustion were given respectively. After treatment, the expressions of TGF-β and Smad4 proteins in rat’s colon were detected by using immunohistochemistry (IHC); the expression of Smad4 mRNA was determined by using fluorescence quantitative polymerase chain reaction (FQ-PCR). Results: Compared with the normal group, the expressions of TGF-β protein, Smad4 protein and Smad4 mRNA significantly increased in the model group (P<0.01). Compared with the model group, the expressions of TGF-β protein, Smad4 protein, and Smad4 mRNA dropped markedly (P<0.01 or P<0.05) after treatments of mild moxibustion, EA, and herb-partitioned moxibustion. Conclusion: The expressions of TGF-β protein, Smad4 protein and Smad4 mRNA significantly increase in CD rats with intestinal fibrosis; while mild moxibustion, EA and herb-partitioned moxibustion at Tianshu (ST 25) and Qihai (CV 6) all can down-regulate the abnormal expressions.
Alternative Title
J Acupunct Tuina Sci
Creator
Date
Language
English
issn
1672-3597
issue
6
page end
335
page start
331
volume
10