Regulatory effect of electroacupuncture on ceramide galactosyltransferase expression in sciatic nerve of experimental diabetic rats

Item

Title

Regulatory effect of electroacupuncture on ceramide galactosyltransferase expression in sciatic nerve of experimental diabetic rats

Description

J Acupunct Tuina Sci (2014), Chao, Jiang; Ping, Liu; Yun-yun, Zhang; Qiu-juan, Zhang; Gao-min, Huang.

Abstract

Objective: To observe the change of ceramide galactosyltransferase (CGT) expression in sciatic nerve of experimental diabetic rats after electroacupuncture (EA) treatment. Methods: The rat model of diabetic peripheral neuropathy (DPN) was established with Streptozotocin (STZ). Twenty-eight rats were randomly divided into a normal group, a model group, an EA acupoint group, and an EA non-acupoint group, with 7 rats in each group. Acupuncture on points Shenshu (BL 23) and Zusanli (ST 36) was performed in the EA group using EA electronic device with continuous wave, 2 Hz, 20 min every time, once every other day for 12 times. Acupuncture (on the tip of rat tail) in EA non-acupoint group was performed with the same electronic parameters and time. The model group and the normal group were not given any treatment, except the same fixation as that in the EA acupoint group. After 12 treatments, the albumen and mRNA expressions of CGT of sciatic nerve in rats from each group were measured with real-time polymerase chain reaction (RT-PCR), fluorescence quantitative PCR (FQ-PCR) and immunohistochemical methods. Results: After modeling, the CGT expression was significantly lower than that in the normal group (P<0.01); however, after 12 EA treatments, the CGT positive cells in the EA acupoint group were up-regulated and significantly higher than those in the EA non-acupoint group and the model group (P<0.01). The CGT mRNA expression of the rats after modeling was also higher than that of the normal rats (P<0.01), and the expression in the EA acupoint group was significantly lower than those in the model group and the EA non-acupoint group (P<0.01, P<0.05). Conclusion: EA treatment with acupoints displays some therapeutic or preventive effect in the recovery of DPN via reversing the abnormal expression of CGT mRNA in sciatic nerves of experimental diabetic neuropathy rats, and it is worthy of further study.

Alternative Title

J Acupunct Tuina Sci

Creator

Date

Language

English

Source

Subject

doi

https://doi.org/10.1007/s11726-014-0737-2

issn

1672-3597

issue

1

page end

7

page start

1

volume

12

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