Effect of moxibustion on the motility, absorption, and activities of atpase in small intestine of spleen-deficiency rats

Item

Title

Effect of moxibustion on the motility, absorption, and activities of atpase in small intestine of spleen-deficiency rats

Description

J Acupunct Tunie Sci (2014), Yan, Peng

Abstract

Objective: To observe the effect of moxibustion on intestinal propulsion rate, content of serum D-xylose, content of adenosine triphosphate (ATP) and activities of adenosine triphosphatases (ATPase) in jejunum tissues of spleen-deficiency rats, and to explore the effect of moxibustion on the production of ATP and activities of membrane proteins, for analyzing the mechanism of moxibustion in reinforcing the spleen-stomach. Methods: Forty healthy Sprague Dawley rats were randomly divided into four groups, namely group A (blank control group), group B (model control group), group C (moxibustion group), and group D (herbs group). The animal model of spleen-deficiency was established by intragastric administration with Da Huang (Radix et Rhizoma Rhei) infusion. The rats in group C received moxibustion at Zusanli (ST 36), Zhongwan (CV 12), Guanyuan (CV 4), Pishu (BL 20), and Weishu (BL 21). The rats in group D received intragastric administration with Si Jun Zi decoction. The intestinal propulsion was measured by toner method, and the content of D-xylose in serum was detected by phloroglucinol method. Colormetry method was used to detect the content of ATP and activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in jejunum tissues. Results: Compared with group A, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATP in jejunum tissues decreased significantly in group B (p<0.05 or p<0.01). Compared with group B, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in jejunum tissues increased significantly in group C and D (p<0.05 or p<0.01), while there was no significant difference between group C and group D. Conclusion: Moxibustion can increase the intestinal propulsion rate, content of D-xylose in serum, and ATP in jejunum tissues, as well as the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase of spleen-deficiency rats, which suggests that moxibustion can enhance the motility and absorption functions of small intestine by promoting the production of ATP in intestinal epithelial cells, increasing the content of membrane proteins, and improving transmembrane transport.

Alternative Title

J Acupunct Tuina Sci

Creator

Date

Language

English

Source

Subject

issn

1672-3597

issue

2

page end

72

page start

67

volume

12

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